Design, synthesis and properties of hydrogen peroxide fluorescent probe based on benzothiazole

Abnormal intracellular hydrogen peroxide (H2O2) concentration is related to cancer, cardiovascular, cerebrovascular diseases and other diseases. A novel H2O2 fluorescent probe (BBS) was designed and synthesized. The fluorescence intensity shows good linear relationship with the concentration of H2O2 (0-40 μM), and the detection limit is 132 nM. The response mechanism of probe BBS was verified and analyzed by UV-Vis spectra, titration spectra and density functional theory (DFT) on Gaussian 09 programme. BBS can be used for bioimaging in living cells.