Radiation Induced Late Damage to the Barrier Function of Small Blood Vessels in Mouse Bladder

No AccessJournal of UrologyInvestigative urology1 Dec 2006Radiation Induced Late Damage to the Barrier Function of Small Blood Vessels in Mouse Bladder Jana Jaal, and Wolfgang Dörr Jana JaalJana Jaal Department of Radiotherapy and Radiation Oncology, Medical Faculty Carl Gustav Carus, University of Technology of Dresden, Dresden, Germany , and Wolfgang DörrWolfgang Dörr Experimental Centre, Medical Faculty Carl Gustav Carus, University of Technology of Dresden, Dresden, Germany View All Author Informationhttps://doi.org/10.1016/j.juro.2006.07.138AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We identified changes in vascular barrier function in relation to collagen deposition during the late radiation response of mouse bladders. In this study albumin leakage was assessed as a marker of blood vessel barrier disruption. Materials and Methods: Female C3H/Neu mice were irradiated with a single dose of 20 Gy and sacrificed for (immuno) histological studies after 90, 120, 180, 240 and 360 days. The fractional area of subepithelial tissue with extravascular albumin signal was defined. Moreover, the amount of collagen was determined after Masson's trichrome staining on an arbitrary score of 0 to 3. Results: Subepithelial albumin infiltration due to leakage from the blood vessels was seen in irradiated animals during the entire late phase. It was most pronounced at days 120 and 180 after treatment (vs control p <0.0001). Similarly an increase in collagen deposition in the bladder wall was seen during the whole late radiation phase but with increasing values toward the end of the experiment (day 360 vs control p <0.0001). Conclusions: Irradiation of the bladder results in late damage to the barrier function of small subepithelial blood vessels, causing pronounced albumin leakage at the early steps of the chronic radiation reaction, clearly preceding excessive collagen deposition in the bladder wall. Current data suggest a vascular component in the development of late radiation induced changes in the bladder. References 1 : Radiation response and tolerance of normal tissues. In: Basic Clinical Radiobiology. Edited by . London: Arnold2002: 40. chapt. 4. Google Scholar 2 : The short-term effect of pelvic irradiation for gynecologic malignancies on bladder function. Obstet Gynecol1987; 70: 81. Google Scholar 3 : Radiation induced inflammatory changes in the mouse bladder: the role of cyclooxygenase-2. J. Urol2006; 175: 1529. Link, Google Scholar 4 : Early and long term effects of radiation on ICAM-1 expression in mouse urinary bladder endothelium. Int J Radiat Biol2005; 81: 387. 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Volume 176Issue 6December 2006Page: 2696-2700 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordsblood vesselsserum albuminradiotherapyfibrosisbladderMetricsAuthor Information Jana Jaal Department of Radiotherapy and Radiation Oncology, Medical Faculty Carl Gustav Carus, University of Technology of Dresden, Dresden, Germany More articles by this author Wolfgang Dörr Experimental Centre, Medical Faculty Carl Gustav Carus, University of Technology of Dresden, Dresden, Germany More articles by this author Expand All Advertisement PDF DownloadLoading ...