电穿孔
枯草芽孢杆菌
转化(遗传学)
原生质体
质粒
生物
DNA
微生物学
遗传学
杆菌科
计算生物学
基因
细菌
作者
Diego Romero,Alejandro Pérez‐García,Jan‐Willem Veening,Antonio de Vicente,Oscar P. Kuipers
标识
DOI:10.1016/j.mimet.2006.01.005
摘要
A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.
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