Defective macrophage function in aquaporin‐3 deficiency

The FASEB JournalVolume 25, Issue 12 p. 4233-4239 Research CommunicationFree to Read Defective macrophage function in aquaporin-3 deficiency Na Zhu, Na Zhu Central Research Laboratory, Bethune Second Hospital, Changchun, China Biotech Center for Viral Disease Emergency, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaSearch for more papers by this authorXuechao Feng, Xuechao Feng Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorChengyan He, Chengyan He China-Japan Union Hospital, Jilin University, Changchun, ChinaSearch for more papers by this authorHongwen Gao, Hongwen Gao Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorLongfei Yang, Longfei Yang Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorQingjie Ma, Qingjie Ma China-Japan Union Hospital, Jilin University, Changchun, ChinaSearch for more papers by this authorLei Guo, Lei Guo Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorYing Qiao, Ying Qiao Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorHong Yang, Hong Yang Central Research Laboratory, Bethune Second Hospital, Changchun, China School of Life Science, Liaoning Normal University, Dalian, ChinaSearch for more papers by this authorTonghui Ma, Corresponding Author Tonghui Ma [email protected] Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaCorrespondence: Central Research Laboratory, Bethune Second Hospital of Jilin University, Changchun, 130041, P.R. China. E-mail: [email protected]Search for more papers by this author Na Zhu, Na Zhu Central Research Laboratory, Bethune Second Hospital, Changchun, China Biotech Center for Viral Disease Emergency, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaSearch for more papers by this authorXuechao Feng, Xuechao Feng Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorChengyan He, Chengyan He China-Japan Union Hospital, Jilin University, Changchun, ChinaSearch for more papers by this authorHongwen Gao, Hongwen Gao Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorLongfei Yang, Longfei Yang Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorQingjie Ma, Qingjie Ma China-Japan Union Hospital, Jilin University, Changchun, ChinaSearch for more papers by this authorLei Guo, Lei Guo Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorYing Qiao, Ying Qiao Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaSearch for more papers by this authorHong Yang, Hong Yang Central Research Laboratory, Bethune Second Hospital, Changchun, China School of Life Science, Liaoning Normal University, Dalian, ChinaSearch for more papers by this authorTonghui Ma, Corresponding Author Tonghui Ma [email protected] Central Research Laboratory, Bethune Second Hospital, Changchun, ChinaCorrespondence: Central Research Laboratory, Bethune Second Hospital of Jilin University, Changchun, 130041, P.R. China. E-mail: [email protected]Search for more papers by this author First published: 24 August 2011 https://doi.org/10.1096/fj.11-182808Citations: 5Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat ABSTRACT Macrophages play an essential role in innate immunity. We found that mouse resident peritoneal macrophages (mRPMs) express the aquaglyceroporin aquaporin-3 (AQP3) in a plasma membrane pattern. AQP3-deficient (AQP3–/–) mice showed significantly greater mortality than wild-type (AQP3+/+) mice in a model of bacterial peritonitis. To establish the cellular mechanism of the peritonitis phenotype, measurements were made of mRPM phagocytosis, migration, and water/glycerol permeability. We found significantly impaired engulfment of Escherichia coli and chicken erythrocytes in AQP3–/– vs. AQP3+/+ mRPMs, as well as impaired migration of AQP3–/– mRPMs in response to a chemotactic stimulus. In AQP3+/+ mRPMs, AQP3 was polarized to pseudopodia at the leading edge during migration and around the phagocytic cup during engulfment. Water and glycerol permeabilities in mRPMs from AQP3–/– mice were reduced compared to mRPMs from AQP3+/+ mice. Cellular glycerol and ATP content were remarkably lower in AQP3–/– vs. AQP3+/+ mRPMs, and glycerol supplementation partially rescued the reduced ATP content and impaired function of AQP3–/– mRPMs. These data implicate AQP3 as a novel determinant in macrophage immune function by a cellular mechanism involving facilitated water and glycerol transport, and consequent phagocytic and migration activity. This is the first study demonstrating involvement of an aquaporin in innate immunity. Our results suggest AQP3 as a novel therapeutic target in modulating the immune response in various infectious and inflammatory conditions.—Zhu, N., Feng, X., He, C., Gao, H., Yang, L., Ma, Q., Guo, L., Qiao, Y., Yang, Y., Ma, T. Defective macrophage function in aquaporin-3 deficiency. FASEB J. 25, 4233–4239 (2011). www.fasebj.org Citing Literature Volume25, Issue12December 2011Pages 4233-4239 RelatedInformation