Immobilized Fullerene C60‐Enzyme‐Based Electrochemical Glucose Sensor

A mixed-valence cluster of cobalt(II) hexacyanoferrate and fullerene C60-enzyme-based electrochemical glucose sensor was developed. A water insoluble fullerene C60-glucose oxidase (C60-GOD) was prepared and applied as an immobilized enzyme on a glassy carbon electrode with cobalt(II) hexacyanoferrate for analysis of glucose. The glucose in 0.1 M KCl/phosphate buffer solution at pH = 6 was measured with an applied electrode potential at 0.0 mV (vs Ag/AgCl reference electrode). The C60-GOD-based electrochemical glucose sensor exhibited efficient electro-catalytic activity toward the liberated hydrogen peroxide and allowed cathodic detection of glucose. The C60-GOD electrochemical glucose sensor also showed quite good selectivity to glucose with no interference from easily oxidizable biospecies, e.g. uric acid, ascorbic acid, cysteine, tyrosine, acetaminophen and galactose. The current of H2O2 reduced by cobalt(II) hexacyanoferrate was found to be proportional to the concentration of glucose in aqueous solutions. The immobilized C60-GOD enzyme-based glucose sensor exhibited a good linear response up to 8 mM glucose with a sensitivity of 5.60 × 102 nA/mM and a quite short response time of 5 sec. The C60-GOD-based glucose sensor also showed a good sensitivity with a detection limit of 1.6 × 10-6 M and a high reproducibility with a relative standard deviation (RSD) of 4.26%. Effects of pH and temperature on the responses of the immobilized C60-GOD/cobalt(II) hexacyanoferrate-based electrochemical glucose sensor were also studied and discussed.