达帕格列嗪
化学
色谱法
甲酸
电喷雾电离
质谱法
糖尿病
2型糖尿病
内分泌学
医学
作者
Anne‐Françoise Aubry,Huidong Gu,Reynald Magnier,Ling Morgan,Xiaohui Xu,Mark A. Tirmenstein,Bonnie Wang,Yuzhong Deng,Jinnan Cai,Philippe Couerbe,Mark E. Arnold
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2010-12-01
卷期号:2 (12): 2001-2009
被引量:46
摘要
Background: Dapagliflozin is an inhibitor of sodium-glucose co-transporter 2 (SGLT-2) in development for the treatment of Type 2 diabetes. To support toxicology studies, LC–MS/MS methods were developed and validated for the quantitation of dapagliflozin in rat plasma. Results: The assay uses solid phase extraction and LC–MS/MS analysis in negative ion electrospray ionization mode. Because dapagliflozin readily forms adducts in the presence of formic acid, the mobile phases were simple mixtures of water and acetonitrile. The assay was validated in the concentration range of 5–2000 ng/ml with good intra- and inter-day precisions and acceptable sample stability. Conclusion: The validated assay was successfully applied to the quantitation of dapagliflozin in plasma in support of preclinical studies in both normal and diabetic rats.
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