Immune mechanisms in periodontitis � effect of IL-4 gene polymorphisms on cytokine production

Event Abstract Back to Event Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production Jirina Bartova1*, Petra Borilova Linhartova2, Stepan Podzimek1, Kazi Svobodová1, Tatjana Janatova1, Antonín Fassmann3, Jana Duskova1 and Lydie Izakovicova-Holla2 1 Institute of Clinical and Experimental Dental Medicine , GUH and First Faculty of Medicine Charles University, Czechia 2 Faculty of Medicine Masaryk University, Department of Physiology, Czechia 3 Clinic of Stomatology, Faculty of Medicine MU and St Anne´s Hospital, Czechia Introduction: Periodontitis is a chronic inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria and immune mechanisms play important roles. The aim of this study was to assess the influence of IL-4 gene polymorphisms on cytokine production Methods: Cytokine production (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-8 , IL-10, TNF–α, INF–γ) was studied after in vitro stimulation of isolated peripheral blood mononuclear cells (PBMC) by mitogens and dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, Tannerrella forsythensis, Porphyromonas gingivalis and Heat Shock Protein 60). Cytokine production was detected by multiplex cytokine analysis system Luminex. The results were correlated with IL-4 genotypes in patients with chronic periodontitis (CP) and healthy controls. Results: We found that mononuclear cells isolated from peripheral blood of CP patients with investigated IL-4 polymorphisms (-589C/T, -33C/T, VNTR in intron 3) significantly changed production of IFN-, IL-10, IL-1 and IL-1, TNF-, IL-10, MCP-1, IL-6 after stimulation by all selected bacteria and HSP (p0.05). Conclusion: In patients with CP, IL-4 polymorphisms may influence the function of mononuclear cells to produce not only IL-4 but also other cytokines. The results of this study support Seymour’s hypothesis (1999) about the role of TH2 produced cytokines such as IL-4 in genetic predisposition to chronic periodontitis. Acknowledgements The study was supported by PRVOUK-P28/LF1/6 (Ministry of Education, Youth and Sports, Czech Republic) and by grant IGA NT11405-6. Keywords: Periodontitis, immune mechanisms, production of cytokines, IL-4, IL-4 gene polymorphismus Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Bartova J, Borilova Linhartova P, Podzimek S, Svobodová K, Janatova T, Fassmann A, Duskova J and Izakovicova-Holla L (2013). Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00278 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Jirina Bartova, Institute of Clinical and Experimental Dental Medicine , GUH and First Faculty of Medicine Charles University, Prague, Czechia, jirina.bartova@post.cz Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Kazi Svobodová Tatjana Janatova Antonín Fassmann Jana Duskova Lydie Izakovicova-Holla Google Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Kazi Svobodová Tatjana Janatova Antonín Fassmann Jana Duskova Lydie Izakovicova-Holla Google Scholar Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Kazi Svobodová Tatjana Janatova Antonín Fassmann Jana Duskova Lydie Izakovicova-Holla PubMed Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Kazi Svobodová Tatjana Janatova Antonín Fassmann Jana Duskova Lydie Izakovicova-Holla Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.