生物发光
荧光素酶
荧光素
化学
萤火虫协议
生物传感器
萤光素酶类
费斯特共振能量转移
生物发光成像
生物化学
作者
Darya V Smirnova,N. N. Ugarova
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2015-11-03
卷期号:18 (10): 946-951
被引量:4
标识
DOI:10.2174/1386207318666150917095731
摘要
Bioanalytical systems based on the Bioluminescence Resonance Energy Transfer (BRET) are widely used in fundamental biochemical studies, as well as for screening and analysis of biologically active compounds. The Renilla luciferase is the most often used energy donor in this system despite the fact that it has low bioluminescence quantum yield and demonstrates not so stable luminescence in time as the firefly luciferase. Moreover, the bioluminescence λmax is observed in the green region of the spectrum, which complicates signal recording in tissues during in vivo experiments. The firefly luciferases do not have such drawbacks and show great promise for applications in BRET systems. Different versions of BRET systems based on firefly luciferases and the methods for increasing their efficiency are considered in this review; examples of the use of BRET systems based on the firefly luciferases for highly sensitive determination of proteases and for homogeneous immunoassays are presented. Keywords: Luciferase, bioluminescence, luciferase-based assay, bioluminescence resonance energy transfer, BRET.
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