苄基异喹啉
生物碱
异喹啉
酶
限制
化学
生物化学
生物合成
生物
立体化学
机械工程
工程类
作者
Tomoya Takemura,Yit‐Lai Chow,Takehiko Todokoro,Takuya Okamoto,Fumihiko Sato
出处
期刊:Methods in molecular biology
日期:2010-01-01
卷期号:: 95-109
被引量:15
标识
DOI:10.1007/978-1-60761-723-5_7
摘要
Benzylisoquinoline alkaloids are one of the most important groups of secondary metabolites and include the economically important analgesic morphine and the antimicrobial agent berberine. To improve the productivity of these alkaloids, we investigated the effects of putative rate-limiting step enzymes in alkaloid biosynthesis. We constructed several over-expression vectors for biosynthetic enzymes and introduced them into cultured California poppy, a model isoquinoline alkaloid-producing plant. HPLC/LC-MS analysis of transgenic cells revealed that these enzymes varied in their ability to increase alkaloid production. We describe the use of a rate-limiting step gene to improve alkaloid productivity.
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