Isolation and Purification of Dendritic Cells from Human Peripheral Blood

Objective Proliferation and isolation of dendritic cells from human peripheral blood. Methods Monocytes isolated from human peripheral blood, culture for 2 hours. The adherent cells were cultured with different cytokines respectively in serum\|free culture. The cells were examined by phenotype/FACS for 1,6 and 8 days respectively,and the cell growth was inspected under a light microscope and electronmicroscope at regular interval and determined function assay. Results During 6 days to 8 days, a large number of DC with high purity were generated. DC expressed HLA\|DR,CD\-\{40\},CD\-\{86\} and CD\-\{83\} costimulating moleculars highly on cell surface and could stimulate proliferation of allogeneic T lymphocytes. Conclusion In serum\|free culture,a numbers of dendritic cells was obtained from the human peripheral blood.\;