A Mitochondria‐Specific Orange/Near‐Infrared‐Emissive Fluorescent Probe for Dual‐Imaging of Viscosity and H2O2 in Inflammation and Tumor Models

Main observation and conclusion Elucidating the intrinsic relationship between viscosity/H 2 O 2 and mitochondria‐associated diseases remains a great challenge owing to the lack of research on multiple diseases models, such as inflammation and malignant tumor models. In this work, we have developed a mitochondria‐specific orange/near‐infrared‐emissive fluorescent probe TTPB, for dual‐imaging of viscosity and H 2 O 2 levels in two different channels. The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm, and was highly sensitive to H 2 O 2 in orange channel with emission peak at 586 nm. Moreover, TTPB has good mitochondria‐specific ability and permits individual detecting of viscosity in NIR channels and H 2 O 2 levels in orange channel in living cells. More notably, TTPB was successfully applied to simultaneously image the viscosity and H 2 O 2 levels in inflammation and cancer models.