环介导等温扩增
核糖核酸
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
检出限
2019年冠状病毒病(COVID-19)
劈理(地质)
计算生物学
化学
生物
病毒学
计算机科学
纳米技术
DNA
色谱法
材料科学
医学
生物化学
基因
传染病(医学专业)
病理
疾病
古生物学
断裂(地质)
作者
Chao Zhang,Tingting Zheng,Hongliang Fan,Tao Zhang,Da Han
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2021-02-26
卷期号:4 (5): 3805-3810
被引量:8
标识
DOI:10.1021/acsabm.0c01674
摘要
Rapid detection of SARS-CoV-2 RNA is critical for reducing the global transmission of COVID-19. Here, we report a simple and versatile assay for detection of SARS-CoV-2 RNA based on aligner-mediated cleavage-based strand displacement amplification (AMC-SDA). The entire amplification procedure takes less than 25 min without professional instruments or requirement of specific target sequences and can reach a limit of detection of attomolar RNA concentration. Using pseudovirus as mimicry of clinical SARS-CoV-2 positive samples, we achieved a diagnostic accuracy of 100% in 10 simulated samples (five positive and five negative). We anticipate that our method will provide a universal platform for rapid and accurate detection of emerging infectious diseases.
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