环介导等温扩增
多路复用
病毒学
生物
底漆(化妆品)
冠状病毒
分子生物学
微生物学
2019年冠状病毒病(COVID-19)
医学
化学
遗传学
DNA
疾病
有机化学
病理
传染病(医学专业)
作者
Woong Bi Jang,Da Hye Lim,Jung Hyun Yoon,Ahran Kim,임민섭 ( Minsup Lim ),Jeonghun Nam,Richard Yanagihara,Sook Won Ryu,Bo Hyun Jung,Namhee Ryoo,Chae Seung Lim
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2021-03-03
卷期号:16 (3): e0248042-e0248042
被引量:32
标识
DOI:10.1371/journal.pone.0248042
摘要
A newly identified coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), has spread rapidly from its epicenter in China to more than 150 countries across six continents. In this study, we have designed three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primer sets to detect the RNA-dependent RNA polymerase (RdRP), Envelope (E) and Nucleocapsid protein (N) genes of SARS CoV-2. For one tube reaction, the detection limits for five combination SARS CoV-2 LAMP primer sets (RdRP/E, RdRP/N, E/N, RdRP/E/N and RdRP/N/Internal control (actin beta)) were evaluated with a clinical nasopharyngeal swab sample. Among the five combination, the RdRP/E and RdRP/N/IC multiplex LAMP assays showed low detection limits. The sensitivity and specificity of the RT-LAMP assay were evaluated and compared to that of the widely used Allplex™ 2019-nCoV Assay (Seegene, Inc., Seoul, South Korea) and PowerChek™ 2019-nCoV Real-time PCR kit (Kogenebiotech, Seoul, South Korea) for 130 clinical samples from 91 SARS CoV-2 patients and 162 NP specimens from individuals with (72) and without (90) viral respiratory infections. The multiplex RdRP (FAM)/N (CY5)/IC (Hex) RT-LAMP assay showed comparable sensitivities (RdRP: 93.85%, N: 94.62% and RdRP/N: 96.92%) to that of the Allplex™ 2019-nCoV Assay (100%) and superior to those of PowerChek™ 2019-nCoV Real-time PCR kit (RdRP: 92.31%, E: 93.85% and RdRP/E: 95.38%).
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