The piezoelectric gel-fiber-particle substrate containing short PVDF-chitosan-gelatin nanofibers and mesoporous silica nanoparticles with enhanced antibacterial activity as a potential of wound dressing applications

Self-electrical stimuli substrate with the structure of gel-fiber-particles to make an electrical perimeter in contact with skin wound would provide a new outline for wound dressing applications. To fabricate such self-electrical stimuli wound dressing substitute, chopped poly(vinylidene fluoride) (PVDF)-chitosan-gelatin nanofibers and MCM41 nanoparticles were designated. Designed nanostructures were incorporated in gellan-poly(vinyl alcohol) (PVA). The morphology of chopped fibers and nanoparticles was studied via scanning electron microscopy. Chemical characterization was performed with the help of the Fourier transforms infrared spectra method, the mesoporous texture and particle size of synthesized nanoparticles are analyzed using Barrett–Joyner–Halenda and dynamic light scattering methods. The swelling ratio, antibacterial and MTT assays are the characterization methods of the synthesized wound dressing. Our results demonstrate that chopped PVDF-chitosan-gelatin nanofibers were fabricated and mesoporous silica nanoparticles with the average particle size of 50 nm and cavity volume of 0.61 cm3/g were synthesized. Gellan-PVA incorporated short nanofibers and silica nanoparticles showed enhanced electrical conductivity and crystallinity behavior. Enhanced antibacterial activity and cellular behavior were observed in gel-containing fibers and particles. Obtained results suggest the novel wound dressing materials based on the idea of self-electrical stimuli gel-fiber-particles structure as a possible candidate for wound healing.