Application an edible active coating based on chitosan- Ferulago angulata essential oil nanoemulsion to shelf life extension of Rainbow trout fillets stored at 4 °C

The present study was undertaken to evaluate the feasibility of nanoemulsification for improving the efficiency of chitosan- Ferulago angulata essential oil coating (CH + EO) on extending the shelf life of Rainbow trout fillets during 16 days storage at 4 °C. The average droplet size of coarse emulsions was dramatically reduced after nanoemulsification which was accompanied by a significantly more positive ζ-potential and lower polydispersity index. In vitro antibacterial potential of CH + EO against two fish specific spoilage organisms, Shewanella putrefaciens and Pseudomonas fluorescens, and its antioxidant activity improved significantly (p < 0.05) when nanoemulsions were fabricated. The CH + EO nanoemulsion (3% EO) treatment exhibited a significantly better inhibitory effect on bacterial growth of refrigeration stored Rainbow trout fillets. Moreover, nanoemulsification potentiated the efficacy of CH + EO in retarding the increase of TVB-N and lipid peroxidation in fish fillets. Texture, colour, and overall acceptability of CH + EO nanoemulsion treated samples were significantly (p < 0.05) better than those of other samples. Results suggest nanoemulsification as a potential approach to enhance the efficacy of the active coating.