QbD based exploration of resveratrol loaded polymeric micelles based carbomer gel for topical treatment of plaque psoriasis: In vitro, ex vivo and in vivo studies

Topical delivery of Resveratrol is a safe method for treatment of plaque psoriasis which could overcome the existing limitations. However, poor permeability of biomolecules through the scaly and inflamed psoriatic skin is the major challenge to develop an effective and safe topical dosage form. Resveratrol loaded polymeric micelles of (PM) were developed using QbD based approach employing risk assessment and Central Composite Design to get optimized CMAs to achieve desired QTPP and CQAs (i.e. particle size, micellar incorporation efficiency, and skin deposition) which were obtained successfully. The optimized PM was converted into a gel (PMG) and evaluated for morphology and other physicochemical properties. It was also evaluated for ex vivo skin permeation and deposition ability, in vitro antioxidant activity, and in vivo efficacy using IMQ-induced psoriatic-like plaque model in mice. In vivo studies ratified the significant effectiveness of PMG as compared to conventional gel (CG). A significant reduction in PASI score, splenomegaly, serum cytokines levels, and hyperkeratosis in PMG treated group indicated superior utility of Resveratrol loaded PMG formulation. The present investigation ratifies QbD driven development of polymeric micelles which may be explored for better delivery of encapsulated cargos at the target site with enhanced therapeutic and dermatological benefits.