芳香族L-氨基酸脱羧酶
酪氨酸羟化酶
色氨酸羟化酶
芳香族氨基酸
单胺类神经递质
生物化学
血清素
多巴胺
酶
酪氨酸
苯丙氨酸羟化酶
氨基酸
色氨酸
苯丙氨酸
生物
化学
内分泌学
受体
5-羟色胺能
作者
Alessandro Groaz,Silvia Galvan,Luca Valer,Daniele Rossetto,Filippo Benedetti,Graziano Guella,Ö. Duhan Toparlak,Sheref S. Mansy
标识
DOI:10.1002/adbi.202000118
摘要
Abstract The synthesis of serotonin and dopamine with purified enzymes is described. Both pathways start from an amino acid substrate and synthesize the monoamine neurotransmitter in two enzymatic steps. The enzymes human tryptophan hydroxylase isoform 2, Rattus norvegicus tyrosine hydroxylase, Chlamydia pneumoniae Cpn1046, and aromatic amino acid decarboxylase from Drosophila melanogaster are recombinantly expressed, purified, and shown to be functional in vitro. The hydroxylases efficiently convert L‐DOPA (L‐dihydroxy‐phenylalanine) and 5‐HTP (5‐hydroxytryptophan) from L‐tyrosine and L‐tryptophan, respectively. A single aromatic amino acid decarboxylase is capable of converting both hydroxylated intermediates into the final neurotransmitter. The platform described here may facilitate future efforts to generate medically useful artificial cells and nanofactories.
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