亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Astaxanthin protects mesenchymal stem cells from oxidative stress by direct scavenging of free radicals and modulation of cell signaling

虾青素 间充质干细胞 氧化应激 细胞生物学 激进的 细胞 化学 清除 干细胞 细胞信号 信号转导 生物 生物化学 抗氧化剂 类胡萝卜素
作者
Saeed Mohammadi,Abolfazl Barzegari,Alireza Dehnad,Jaleh Barar,Yadollah Omidi
出处
期刊:Chemico-Biological Interactions [Elsevier]
卷期号:333: 109324-109324 被引量:16
标识
DOI:10.1016/j.cbi.2020.109324
摘要

Recent evidence has shown that mesenchymal stem cells (MSCs) play vital roles in cell therapy of ischemia/hypoxia damaged tissues. However, after the transplantation, they might undergo apoptosis due to oxidative stress. Thus, some strategies have been developed to support stem cells in harsh conditions, including pre-treatment of the cells with antioxidants. Of various antioxidants, in this study, astaxanthin (ATX) was used to protect adipose-derived MSCs against oxidative stress. The MSCs were exposed to different doses of hydrogen peroxide, and then the expression of key genes involved in the redox signaling pathway was studied, including nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NADPH quinine oxidoreductase 1 (NQO1). The balance of intracellular reactive oxygen species was detected with the H2DCFDA molecular probe. Additionally, for the detection of apoptosis and protective effect of ATX, the DAPI/Phallacidin and annexin V cell staining were performed. The results of cellular studies revealed that ATX reduced the H2O2-induced cell apoptosis and oxidative stress. Furthermore, after the induction of oxidative stress, the cells' native antioxidants (HO-1 and NQO1) were overexpressed but they were modulated with ATX treatments (p < 0.023). Based on our findings, ATX could increase the expression of Nrf2 as a key transcription factor of antioxidant enzymes (p < 0.05). These findings support the notion that ATX can act as an effective antioxidant in the pre-treatment of MSCs before cell therapy. Thus, to enhance the viability of stem cells during the transplantation in harsh conditions, the concurrent use of ATX in cell therapy modalities is proposed.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
花开发布了新的文献求助10
3秒前
香蕉觅云应助花开采纳,获得10
12秒前
我是老大应助拟好采纳,获得10
47秒前
caohuijun发布了新的文献求助10
1分钟前
Tinlie完成签到,获得积分20
1分钟前
1分钟前
拟好发布了新的文献求助10
1分钟前
寻道图强应助拟好采纳,获得30
2分钟前
2分钟前
3分钟前
3分钟前
cyb完成签到,获得积分10
4分钟前
iuv完成签到,获得积分10
4分钟前
Lucas应助容若采纳,获得10
4分钟前
4分钟前
中央完成签到,获得积分10
4分钟前
5分钟前
四夕发布了新的文献求助30
5分钟前
小蘑菇应助容若采纳,获得10
5分钟前
从容的盼晴完成签到,获得积分10
5分钟前
中中中完成签到 ,获得积分10
7分钟前
积极的中蓝完成签到 ,获得积分10
8分钟前
Wei发布了新的文献求助10
8分钟前
科研通AI2S应助Wei采纳,获得10
8分钟前
8分钟前
Meimei发布了新的文献求助20
8分钟前
情怀应助陈媛采纳,获得10
10分钟前
10分钟前
Meimei完成签到,获得积分10
10分钟前
陈媛发布了新的文献求助10
10分钟前
爱听歌的大地完成签到 ,获得积分10
11分钟前
荀煜祺完成签到,获得积分10
11分钟前
11分钟前
完美世界应助残酷日光采纳,获得10
13分钟前
去去去去发布了新的文献求助10
13分钟前
孙旭完成签到 ,获得积分10
13分钟前
完美世界应助Ying采纳,获得10
13分钟前
14分钟前
14分钟前
容若发布了新的文献求助10
14分钟前
高分求助中
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Chen Hansheng: China’s Last Romantic Revolutionary 500
宽禁带半导体紫外光电探测器 388
Case Research: The Case Writing Process 300
Global Geological Record of Lake Basins 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3142692
求助须知:如何正确求助?哪些是违规求助? 2793563
关于积分的说明 7806988
捐赠科研通 2449831
什么是DOI,文献DOI怎么找? 1303518
科研通“疑难数据库(出版商)”最低求助积分说明 626959
版权声明 601328