拉布
叶尖生长
花粉管
拟南芥
生物
GTP酶
小泡
细胞生物学
高尔基体
胞吐
拟南芥
内体
花粉
植物
生物化学
细胞内
突变体
分泌物
基因
膜
内质网
授粉
作者
Yuelong Zhou,Yang Yang,Yue Niu,Tingting Fan,Dong Qian,Changxin Luo,Yumei Shi,Shanwei Li,Lizhe An,Yun Xiang
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2020-08-18
卷期号:32 (10): 3170-3187
被引量:43
摘要
RabA4 subfamily proteins, the key regulators of intracellular transport, are vital for tip growth of plant polar cells, but their unique distribution in the apical zone and role in vesicle targeting and trafficking in the tips remain poorly understood. Here, we found that loss of Arabidopsis (Arabidopsis thaliana) AMINOPHOSPHOLIPID ATPASE 3 (ALA3) function resulted in a marked decrease in YFP-RabA4b/ RFP-RabA4d- and FM4-64-labeled vesicles from the inverted-cone zone of the pollen tube tip, misdistribution of certain intramembrane compartment markers, and an obvious increase in pollen tube width. Additionally, we revealed that phosphatidylserine (PS) was abundant in the inverted-cone zone of the apical pollen tube in wild-type Arabidopsis and was mainly colocalized with the trans-Golgi network/early endosome, certain post-Golgi compartments, and the plasma membrane. Loss of ALA3 function resulted in loss of polar localization of apical PS and significantly decreased PS distribution, suggesting that ALA3 is a key regulator for establishing and maintaining the polar localization of apical PS in pollen tubes. We further demonstrated that certain Rab GTPases colocalized with PS in vivo and bound to PS in vitro. Moreover, ALA3 and RabA4d collectively regulated pollen tube growth genetically. Thus, we propose that the tip-localized PS established by ALA3 is crucial for Rab GTPase-mediated vesicle targeting/trafficking and polar growth of pollen tubes in Arabidopsis.
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