长春新碱
粘合连接
并行传输
细胞生物学
势垒函数
生物物理学
化学
细胞粘附
细胞
生物
生物化学
钙粘蛋白
膜
磁导率
作者
Satoshi Konishi,Tomoki Yano,Hiroo Tanaka,Tomoaki Mizuno,Hatsuho Kanoh,Kazuto Tsukita,T. Namba,Atsushi Tamura,Shigenobu Yonemura,Shimpei Gotoh,Hisako Matsumoto,Toyohiro Hirai,Sachiko Tsukita
出处
期刊:Life science alliance
[Life Science Alliance]
日期:2019-08-01
卷期号:2 (4): e201900414-e201900414
被引量:15
标识
DOI:10.26508/lsa.201900414
摘要
The paracellular barrier function of tight junctions (TJs) in epithelial cell sheets is robustly maintained against mechanical fluctuations, by molecular mechanisms that are poorly understood. Vinculin is an adaptor of a mechanosensory complex at the adherens junction. Here, we generated vinculin KO Eph4 epithelial cells and analyzed their confluent cell-sheet properties. We found that vinculin is dispensable for the basic TJ structural integrity and the paracellular barrier function for larger solutes. However, vinculin is indispensable for the paracellular barrier function for ions. In addition, TJs stochastically showed dynamically distorted patterns in vinculin KO cell sheets. These KO phenotypes were rescued by transfecting full-length vinculin and by relaxing the actomyosin tension with blebbistatin, a myosin II ATPase activity inhibitor. Our findings indicate that vinculin resists mechanical fluctuations to maintain the TJ paracellular barrier function for ions in epithelial cell sheets.
科研通智能强力驱动
Strongly Powered by AbleSci AI