溶氧素
维罗尼气单胞菌
生物
嗜水气单胞菌
毒力
微生物学
斑马鱼
发病机制
气单胞菌
免疫学
基因
遗传学
细菌
作者
Chao Ran,Chubin Qin,Mingxu Xie,Jinxiong Zhang,Jie Li,Yadong Xie,Yibing Wang,Shuning Li,Lihui Liu,Xiaozhe Fu,Qiang Lin,Ningqiu Li,Mark R. Liles,Chao Ran
标识
DOI:10.1111/1462-2920.14390
摘要
Summary Aeromonas species are ubiquitous inhabitants of freshwater environments, and are responsible for fish motile aeromonad septicemia (MAS). A. hydrophila is implicated as the primary etiologic agent of MAS. Here, we analysed MAS epidemiological data for cyprinid fish in southern China, and found that A. veronii infections dominated. Consistent with this observation, A. veronii isolates were generally more virulent than A. hydrophila isolates when infecting germ‐free zebrafish larvae via continuous immersion challenge. Through in vivo screening of the transposon library of the A. veronii strain Hm091, aerolysin was identified as the key virulence factor. Further results indicated that A. veronii Hm091 aerolysin disrupts the intestinal barrier of zebrafish, enabling systematic invasion by not only A. veronii Hm091 in a mono‐infection, but also A. hydrophila NJ‐1 in a mixed infection. Moreover, the differences in aerolysin expression and activity were the major contributor to the observed differences between the A. veronii and A. hydrophila strains regarding invasion efficacy via intestine. Together, our results provide new insights into the aetiology and pathogenesis of Aeromonas infections, and highlight the importance of A. veronii ‐targeted treatments in future efforts against MAS.
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