Signal Switch and Signal Amplification for Electrochemical Biosensing

Recently, the investigation on electrochemical biosensing has made great progress. The developed biosensors have extensively been used in different fields, particularly, the sensitive and accurate detection of DNA, protein and metal ions. The designs of specific signal switch and signal amplification strategies are becoming the main topic of electrochemical biosensing research. This presentation will introduce new signal switches and signal amplification strategies developed in last two years. For example, we prepared two electrochemical catalytic probes to design two molecular switches for electrochemical DNA sensing by coupling with porphyrin-encapsulated metal-organic frameworks. Several new signal amplification strategies based on nanotechnology and molecular biology have been presented for DNA detection and immunoassay. By the recognition of nucleic acids to metal ions, some signal switches and label-free methods have been developed for specific and sensitive detection of heavy metal ions. Some new electrochemiluminescent emitters have been synthesized and used for design of new ECL biosensing mechanisms. A wavelength-resolved ratiometric photoelectrochemical technique has also been developed for sensing applications. These works led to a series of amplified methods for sensitive detection of DNA, microRNA and protein. References : 1. L. Cui, J. Wu, H.X. Ju, Biosens. Bioelectron. 79, 861-865 (2016) 2. J. Zhu, X.H. Huo, X.Q. Liu, H.X. Ju,ACS Applied Mater. Interf. 8, 341-349 (2016) 3. P.H. Lin, J.P, Lei, L. Jia, H.X. Ju, Chem. Commun. 52, 1226-1229 (2016) 4. Y.Q. Feng, C.H. Dai, J.P. Lei, H.X. Ju, Y.X. Cheng, Anal. Chem. 88, 845-850 (2016) 5. Q. Hao, J.P. Lei, X.N. Shan, Y. Zang, Q.H. Yang, H.X. Ju, Chem. Sci. 7, 774-780 (2016) 6. Y. Huang, J.P. Lei, Y. Cheng, H.X. Ju, Biosens. Bioelectron. 77, 733-739 (2016) 7. Y. Zang, J.P. Lei, Q. Hao, H.X. Ju, Biosens. Bioelectron. 77, 557-564 (2016) 8. L. Cui, J. Wu, H.X. Ju, Anal. Chem. 87, 10635-10641 (2015) 9. L. Cui, J. Wu, M.Q. Li, H.X. Ju, Elctrochem. Commun. 59, 77-80 (2015) 10. L. Cui, J. Wu, H.X. Ju, Chem Eur. J. 21, 11525-11530 (2015) 11. Y.Q. Feng, Q.B. Wang, J.P. Lei, H.X. Ju, Biosens. Bioelectron. 73, 7-12 (2015) 12. P.H. Ling, J.P. Lei, H.X. Ju, Biosens. Bioelectron. 71, 373–379 (2015) 13. Y. Zang, J.P. Lei, P.H. Ling, H.X. Ju,Anal. Chem. 87, 5430–5436 (2015) 14. P.P. Ling, J.P. Lei, L. Zhang, H.X. Ju, Anal. Chem. 87, 3957–3963 (2015) 15. P.H. Ling, Q. Hao, J.P. Lei, H.X. Ju, J. Mater. Chem. B 3, 1335–1341 (2015) 16. K. Ren, J. Wu, F. Yan, Y. Zhang, H.X. Ju, Biosens. Bioelectron. 66, 345–349 (2015) 17. L. Cui, J. Wu, H.X. Ju, Biosens. Bioelectron. 63, 276-286 (2015)