角膜移植
医学
髓源性抑制细胞
移植
全身给药
免疫学
炎症
淋巴系统
渗透(HVAC)
CD11c公司
淋巴管新生
病理
化学
生物
抑制器
外科
癌症
内科学
转移
生物化学
物理
生物技术
体内
基因
表型
热力学
作者
Jae-young Lee,Honglae Sohn,Chang‐Hyun Kim,Tai‐Gyu Kim,Hyun Soo Lee
出处
期刊:Biomedicines
[MDPI AG]
日期:2022-12-12
卷期号:10 (12): 3223-3223
被引量:4
标识
DOI:10.3390/biomedicines10123223
摘要
Myeloid-derived suppressor cells (MDSCs) are therapeutic agents to prevent graft rejection in organ transplants by modulating inflammation. Herein, the immunosuppressive effect of human cord blood MDSCs on corneal allograft models was confirmed. CB-MDSCs were locally (subconjuctival, 5 × 105) or systemically (intravenous, 1 × 106) injected twice on days 0 and 7. A corneal transplantation model was established using C57BL/6 and BALB/c mice, and corneal graft opacity was measured to evaluate graft rejection up to 6 weeks. Results showed that graft survival in the MDSCs groups increased compared to vehicle groups after 42 days. Systemic and local MDSC administration inhibited the maturation (MHC-IIhi CD11c+) of dendritic cells (DCs) and the differentiation of interferon γ+ CD4+ Th1 in draining lymph nodes (LNs). However, vehicle groups increased the infiltration of CD3+ T cells and F4/80+ macrophages and produced prominent neovascular and lymphatic vessels into the graft site with increased mRNA expression of VEGF-A/C and VEGFR-1/R-3. Local MDSCs administration showed prominent anti-angiogenic/anti-lymphangiogenic effects even at lower MDSCs doses. Thus, CB-MDSCs could relatively suppress the infiltration of pathological T cells/macrophages into the corneas and the migration of mature DCs into draining LNs Therefore, ocular and systemic MDSCs administration showed therapeutic potential for preventing corneal allograft rejection.
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