Gradient electro-processing strategy for efficient conversion of harmful algal blooms to biohythane with mechanisms insight

Globally eruptive harmful algal blooms (HABs) have caused numerous negative effects on aquatic ecosystem and human health. Conversion of HABs into biohythane via dark fermentation (DF) is a promising approach to simultaneously cope with environmental and energy issues, but low HABs harvesting efficiency and biohythane productivity severely hinder its application. Here we designed a gradient electro-processing strategy for efficient HABs harvesting and disruption, which had intrinsic advantages of no secondary pollution and high economic feasibility. Firstly, low current density (0.888-4.444 mA/cm2) was supplied to HABs suspension to harvest biomass via electro-flocculation, which achieved 98.59% harvesting efficiency. A mathematic model considering coupling effects of multi-influencing factors on HABs harvesting was constructed to guide large-scale application. Then, the harvested HABs biomass was disrupted via electro-oxidation under higher current density (44.44 mA/cm2) to improve bioavailability for DF. As results, hydrogen and methane yields of 64.46 mL/ (g VS) and 171.82 mL/(g VS) were obtained under 6 min electro-oxidation, along with the highest energy yield (50.1 kJ/L) and energy conversion efficiency (44.87%). Mechanisms of HABs harvesting and disruption under gradient electro-processing were revealed, along with the conversion pathways from HABs to biohythane. Together, this work provides a promising strategy for efficient disposal of HABs with extra benefit of biohythane production.