蛋白质组
生物
蛋白质组学
线粒体
能量代谢
甘油磷脂
新陈代谢
细胞生物学
生物信息学
生物化学
内分泌学
基因
膜
磷脂
作者
Xiaopeng Chen,Yiyun Liu,Juncai Pu,Siwen Gui,Dongfang Wang,Xiaogang Zhong,Wei‐Yi Chen,Tao Wei,Yue Chen,Xiang Chen,Peng Xie
标识
DOI:10.1016/j.jad.2023.04.031
摘要
The gut-brain axis has been shown to play an important role in depression. However, few studies have examined proteomic changes in the intestine of the nonhuman primate model of depression.We investigated the intestinal proteome of macaques (Macaca fascicularis) with depression-like (DL) behaviors by data-independent acquisition techniques. We also performed integration analyses of proteomic changes, previous metabolomic and microbiotic data. Moreover, we confirmed the gene expressions of key proteins.Sixty-five differentially expressed proteins (DEPs) were identified, of which fifty-four DEPs were down-regulated and the others were altered conversely in DL macaques compared with the control group. Pathway analysis indicated that mitochondrial function and energy metabolism were representative functions of DEPs. The key DEPs were significantly associated with glycerophospholipid metabolism and imbalances of gut microbe. We confirmed that key molecules (NDUFB4, UQCR10, PISD) were significantly inhibited, which may disturb the energy transformation of the electron respiratory chain and the homeostasis of the mitochondrial membrane.Further research is warranted to determine the effects of depression on other peripheral organs.These findings suggest the functional disorder of intestinal mitochondria in DL macaques. The disturbances of glycerophospholipid metabolism and gut microbiota may exacerbate disruptions of energy metabolism. Taking together, our study provides new clues to the relationship between depression and intestinal proteome.
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