胞浆
细胞内
内吞作用
光漂白后的荧光恢复
生物物理学
细胞生物学
程序性细胞死亡
细胞
流式细胞术
细胞凋亡
光漂白
化学
纳米技术
材料科学
荧光
生物
生物化学
膜
免疫学
酶
物理
量子力学
作者
Delong Hou,Yong Xu,Yan Jun,Qi Zeng,Zhonghui Wang,Yi Chen
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-01-23
卷期号:17 (3): 3055-3063
被引量:1
标识
DOI:10.1021/acsnano.2c11876
摘要
Using a photochemically isomerizable cucurbit[6]uril derivative as a building block, we succeeded in generating a large number of oversized 2D materials within the cytosol of a living cell via controlled self-assembly. Fluorescence recovery after a photobleaching assay indicated that the resulting 2D material pieces posed discernible hindrance to not only diffusive spreading but also motor-driven motion of intracellular components in the cytosol, which eventually induced apoptotic cell death. Such behavior was seldom observed in previous 2D material-bearing cells prepared by endocytosis, as the total lateral size constituted by the endocytosed 2D materials per cell failed to exceed a threshold level, leading to a tortuosity of transport path inadequate to impede cytosolic transport in an appreciable manner. By varying the initial concentration of the building block, the existence of such a threshold was experimentally demonstrated from the relationship between the flow cytometry side scatter of the treated cells and corresponding cell viability. With the otherwise well-regulated cytosolic transport dynamics of living cells being physically altered, therapeutics with a new mechanism of action that counteracts drug resistance or intracellular platforms that advance our understanding of subcellular pathology of certain intractable diseases are in sight.
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