赭曲霉毒素A
赭曲霉毒素
硫酸铵沉淀
化学
寡养单胞菌
真菌毒素
酶
代谢物
生物化学
食品科学
生物
微生物学
大小排阻色谱法
16S核糖体RNA
基因
作者
Christoph Gonaus,Laura Wieland,Gerhard G. Thallinger,Shreenath Prasad
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2023-01-01
卷期号:370
被引量:2
标识
DOI:10.1093/femsle/fnad028
摘要
Ochratoxin A is a secondary metabolite that acts as a mycotoxin and is produced by Aspergillus, Penicillium, and other fungal species. It is a threat to animal and human health due to nephrotoxic, carcinogenic, and genotoxic properties and its widespread incidence in agricultural products. To reduce this threat, biological remediation processes are of growing interest. The aerobic gram-negative bacterium Stenotrophomonassp. 043-1a, isolated from soil, was previously shown to degrade ochratoxin A into the non-toxic ochratoxin α and l-phenylalanine (Schatzmayr et al. 2002). However, the enzyme or enzymes catalyzing this reaction in this strain remained elusive. Here, we report the targeted purification of Stenotrophomonassp. 043-1a lysate via ammonium sulfate precipitation, size-exclusion chromatography, and hydrophobic interaction chromatography to identify the ochratoxin A degrading enzymes by subsequent peptide fragment fingerprinting. The metallo-dependent hydrolase Chr1_3858681_3267 and a member of the peptidase S9 family, Chr1_3858681_771, were shown to degrade ochratoxin A. This was, to our knowledge, the first report of an ochratoxin A degrading enzyme from the peptidase S9 family.
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