转移
基因敲除
癌症研究
细胞迁移
帕西林
免疫组织化学
病理
细胞外基质
焦点粘着
生物
组织微阵列
骨肉瘤
细胞
医学
癌症
细胞生物学
细胞培养
信号转导
遗传学
作者
Shiqi Lu,Tong Lü,Jiayu Zhang,Lunbiao Gan,Xinjie Wu,Donghui Han,Keying Zhang,Chao Xu,Haibo Liu,Weijun Qin,Jing Wang,Weihong Wen
出处
期刊:BMC Cancer
[Springer Nature]
日期:2023-03-30
卷期号:23 (1)
被引量:4
标识
DOI:10.1186/s12885-023-10731-7
摘要
Osteosarcoma (OS) is the most common malignant bone tumor with a high incidence in children and adolescents. Frequent tumor metastasis and high postoperative recurrence are the most common challenges in OS. However, detailed mechanism is largely unknown.We examined the expression of CD248 in OS tissue microarrays by immunohistochemistry (IHC) staining. We studied the biological function of CD248 in cell proliferation, invasion and migration of OS cells by CCK8 assay, transwell and wound healing assay. We also studied its function in the metastasis of OS in vivo. At last, we explored the potential mechanism how CD248 promotes OS metastasis by using RNA-seq, western blot, immunofluorescence staining and co-immunoprecipitation using CD248 knockdown OS cells.CD248 was highly expressed in OS tissues and its high expression was correlated with pulmonary metastasis of OS. Knockdown of CD248 in OS cells significantly inhibited cell migration, invasion and metastasis, while had no obvious effect on cell proliferation. Lung metastasis in nude mice was significantly inhibited when CD248 was knocked down. Mechanistically, we found that CD248 could promote the interaction between ITGB1 and extracellular matrix (ECM) proteins like CYR61 and FN, which activated the FAK-paxillin pathway to promote the formation of focal adhesion and metastasis of OS.Our data showed that high CD248 expression is correlated with the metastatic potential of OS. CD248 may promote migration and metastasis through enhancing the interaction between ITGB1 and certain ECM proteins. Therefore, CD248 is a potential marker for diagnosis and effective target for the treatment of metastatic OS.
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