高尿酸血症
尿囊素
尿酸
尿酸氧化酶
生物化学
化学
聚乙二醇化
重组DNA
突变体
蛋白质工程
痛风
酶
分子生物学
生物
基因
聚乙二醇
作者
Mingjie Tong,Shengli Wang,Junqing Luan,Qiuling Xie,Luquan Wang,Xiaoyang Shen,Sheng Xiong
标识
DOI:10.1016/j.ijbiomac.2024.131989
摘要
Uric acid is the end product of purine metabolism in humans due to inactivation of the uricase determined by the mutated uricase gene. Uricase catalyzes the conversion of uric acid into water-soluble allantoin that is easily excreted by the kidneys. Hyperuricemia occurs when the serum concentration of uric acid exceeds its solubility (7 mg/dl). However, modifications to improve the uricase activity is under development for treating the hyperuricemia. Here we designed 7 types of human-porcine chimeric uricase by multiple sequence comparisons and targeted mutagenesis. An optimal human-porcine chimeric uricase mutant (uricase-10) with both high activity (6.33 U/mg) and high homology (91.45 %) was determined by enzyme activity measurement. The engineering uricase was further modified with PEGylation to improve the stability of recombinant protein drugs and reduce immunogenicity, uricase-10 could be more suitable for the treatment of gout and hyperuricemia theoretically.
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