生物
叶绿体
白色(突变)
遗传学
基因
类型(生物学)
植物
生态学
作者
Jie Lan,Qibing Lin,Chunlei Zhou,Xi Liu,Rong Miao,Tengfei Ma,Yaping Chen,Changling Mou,Ruonan Jing,Feng Miao,Thanhliem Nguyen,Yulong Ren,Zhijun Cheng,Xin Zhang,Shijia Liu,Lingxiang Jiang,Jianmin Wan
摘要
Pentatricopeptide repeat (PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice (Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear. Here, we characterized a rice young leaf white stripe (ylws) mutant, which has defective chloroplast development during early seedling growth. Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear- and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2, and rps12, and editing of ndhA, ndhB, and rps14 transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development.
科研通智能强力驱动
Strongly Powered by AbleSci AI