Functional identification of AeHMGR gene involved in regulation of saponin biosynthesis in Aralia elata

生物 皂甙 基因 鉴定(生物学) 生物合成 植物 遗传学 医学 替代医学 病理
作者
Yuxin Xia,Li Ding,Wayne Wei-Ming Dai,Hongfei Liu,Shikai Zhang,Yue Sui,Xiangling You
出处
期刊:Gene [Elsevier]
卷期号:: 148287-148287
标识
DOI:10.1016/j.gene.2024.148287
摘要

Aralia elata (Miq.) Seem, a significant tree species in the Araliaceae family, has medicinal and edible properties. Saponins are the primary active components of A. elata. The 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) is the initial rate-limiting enzyme of the major metabolic pathway of saponins in A. elata. In this study, the AeHMGR gene was identified through screening of transcriptome data. Through the qRT-PCR analysis, it was determined that the expression level of AeHMGR gene is highest in the somatic embryo and stem of A. elata. Heterologous transformation in tobacco revealed that ectopic expression of the AeHMGR gene leads to a significant reduction in the expression levels of the NtSS, NtFPS, and NtSE genes in transgenic tobacco lines, with a minimum expression level of 0.24 times that of the wild type. In the overexpressed callus lines of A. elata, the expression levels of the AeFPS, AeSE, AeSS, and Aeβ-AS genes were also significantly lower compared to the wild type, with a minimum expression level of approximately 0.3 times that of the wild type. Interestingly, the overexpression of the AeHMGR gene in A. elata somatic embryos led to a substantial decrease in the expression levels of AeFPS and AeSS, while the expression levels of AeSE and Aeβ-AS increased. Among the transgenic somatic embryo strain lines, line 7 exhibited the highest expression levels of AeSE and Aeβ-AS, with fold increases of 11.51 and 9.38, respectively, compared with that of the wild-type. Additionally, a high-performance liquid chromatography method was established to detect five individual saponins in transgenic A. elata. The total saponin content in line 7 somatic embryos was 1.14 times higher than that of wild-type materials, but only 0.30 times that of wild-type cultivated leaves. Moreover, the content of oleanolic acid saponin in line 7 was 1.35 times higher than that of wild-type cultivated leaves. These indicate that HMGR can affect triterpene biosynthesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
davyean完成签到,获得积分10
1秒前
hyq008完成签到,获得积分10
1秒前
飞行致幻剂完成签到,获得积分10
1秒前
zhouyou完成签到,获得积分10
2秒前
Shawn完成签到,获得积分10
2秒前
Fxxkme发布了新的文献求助10
3秒前
道友等等我完成签到,获得积分0
3秒前
还是算了完成签到,获得积分10
3秒前
3秒前
陆浩学化学完成签到 ,获得积分10
4秒前
Yyy完成签到 ,获得积分10
4秒前
小白完成签到,获得积分10
4秒前
小灰灰完成签到,获得积分10
5秒前
平常亦凝完成签到,获得积分20
6秒前
花花完成签到,获得积分10
6秒前
waikeyan完成签到,获得积分20
7秒前
不吃了完成签到 ,获得积分10
7秒前
阳光傲菡完成签到 ,获得积分10
8秒前
9秒前
Yziii完成签到,获得积分0
9秒前
平常亦凝发布了新的文献求助10
9秒前
123完成签到 ,获得积分10
10秒前
句号完成签到 ,获得积分10
10秒前
11秒前
碗在水中央完成签到 ,获得积分10
11秒前
TAboo完成签到,获得积分10
11秒前
yoyocici1505完成签到,获得积分10
12秒前
冷酷的啤酒完成签到,获得积分10
12秒前
z小婉完成签到,获得积分10
13秒前
Reybor完成签到,获得积分10
13秒前
高贵的思天完成签到,获得积分10
14秒前
lina完成签到,获得积分10
14秒前
梦璃完成签到,获得积分10
15秒前
追寻思远发布了新的文献求助10
16秒前
Tammy完成签到 ,获得积分10
17秒前
丹阳阳完成签到,获得积分10
17秒前
LVVVB完成签到,获得积分10
18秒前
18秒前
无无完成签到 ,获得积分10
18秒前
射天狼发布了新的文献求助10
19秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3134060
求助须知:如何正确求助?哪些是违规求助? 2784861
关于积分的说明 7769107
捐赠科研通 2440349
什么是DOI,文献DOI怎么找? 1297368
科研通“疑难数据库(出版商)”最低求助积分说明 624959
版权声明 600792