SCI1, a flower regulator of cell proliferation, and its partners NtCDKG2 and NtRH35 interact with the splicing machinery

调节器 细胞生物学 RNA剪接 细胞生长 生长调节剂 生物 植物 遗传学 基因 核糖核酸
作者
Vitor F. Pinoti,Pedro Boscariol Ferreira,Edward J. Strini,Greice Lubini,Vanessa Thomé,Joelma de Oliveira Cruz,Rodrigo Aziani,Andréa C. Quiapim,Andressa Patrícia Alves Pinto,Ana Paula Ulian de Araújo,Henrique C. De Paoli,Maria Cristina da Silva Pranchevicius,Maria Helena S. Goldman
出处
期刊:Journal of Experimental Botany [Oxford University Press]
标识
DOI:10.1093/jxb/erae337
摘要

Abstract Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2–NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2–NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2aʹ, NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2–NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development.
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