生物
发芽
花粉管
延伸率
花粉
黄酮醇
平衡
植物
细胞生物学
生物化学
抗氧化剂
材料科学
授粉
极限抗拉强度
冶金
类黄酮
作者
Anthony Postiglione,A M DeLange,Mohammad Foteh Ali,E. Wang,Maarten Houben,Sinuhe Hahn,Maleana Khoury,Maleana G. Khoury,M Davis,Robert W. Reid,James B. Pease,Ann E. Loraine,Gloria K. Muday
标识
DOI:10.1093/plcell/koae222
摘要
Abstract Elevated temperatures impair pollen performance and reproductive success, resulting in lower crop yields. The tomato (Solanum lycopersicum) anthocyanin reduced (are) mutant harbors a mutation in FLAVANONE 3-HYDROXYLASE (F3H), resulting in impaired flavonol antioxidant biosynthesis. The are mutant has reduced pollen performance and seed set relative to the VF36 parental line, phenotypes that are accentuated at elevated temperatures. Transformation of are with the wild-type F3H gene, or chemical complementation with flavonols, prevented temperature-dependent reactive oxygen species (ROS) accumulation in pollen and restored the reduced viability, germination, and tube elongation of are to VF36 levels. Overexpression of F3H in VF36 prevented temperature-driven ROS increases and impaired pollen performance, revealing that flavonol biosynthesis promotes thermotolerance. Although stigmas of are had reduced flavonol and elevated ROS levels, the growth of are pollen tubes was similarly impaired in both are and VF36 pistils. RNA-seq was performed at optimal and stress temperatures in are, VF36, and the F3H overexpression line at multiple timepoints across pollen tube elongation. The number of differentially expressed genes increased over time under elevated temperatures in all genotypes, with the greatest number in are. These findings suggest potential agricultural interventions to combat the negative effects of heat-induced ROS in pollen that lead to reproductive failure.
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