内生
氨基丁酸
生物化学
γ-氨基丁酸
冷应激
机制(生物学)
酵母
基因
产量(工程)
化学
生物
细胞生物学
认识论
哲学
受体
冶金
材料科学
作者
Tanfang Liu,Yuxing Li,Yanjiao Shi,Jenny Ma,Yuxiao Peng,Xiaocheng Tian,N. Zhang,Fengwang Ma,Cuiying Li
标识
DOI:10.1016/j.ijbiomac.2024.135331
摘要
Low temperatures can seriously affect apple yield and can also cause chilling injury to apple fruit. γ-aminobutyric acid (GABA) plays an important role in improving plant stress resistance. Some studies have reported that GABA can improve cold resistance in plants, only through exogenous treatment; however, the molecular mechanism of its resistance to low temperature is still unknown. This result suggested that exogenous GABA treatment of both apple seedlings and fruit could improve the resistance of apple to low temperatures. MdGAD1, a key gene involved in GABA synthesis, was overexpressed in tomato plants and apple callus to improve their cold tolerance. Both yeast one-hybrid and luciferase assay showed that MdCBF3 could bind to the MdGAD1 promoter to activate its expression and promote GABA synthesis. These results revealed a molecular mechanism utilizing the MdCBF3-MdGAD1 regulatory module that can enhance cold resistance by increasing endogenous GABA synthesis in apple.
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