金属蛋白酶
小干扰RNA
体感系统
细胞
紫杉醇
细胞生物学
背
基因沉默
神经科学
感觉系统
化学
生物
药理学
核糖核酸
基质金属蛋白酶
医学
内科学
解剖
生物化学
基因
化疗
作者
Raquel Tonello,Arthur Silveira Prudente,Sang Hoon Lee,Cinder Faith Cohen,Wenrui Xie,Aditi Paranjpe,Jueun Roh,Chul‐Kyu Park,Gehoon Chung,J.A. Strong,Jun‐Ming Zhang,Temugin Berta
标识
DOI:10.1016/j.bbi.2023.08.005
摘要
Satellite glial cells (SGCs) are among the most abundant non-neuronal cells in dorsal root ganglia (DRGs) and closely envelop sensory neurons that detect painful stimuli. However, little is still known about their homeostatic activities and their contribution to pain. Using single-cell RNA sequencing (scRNA-seq), we were able to obtain a unique transcriptional profile for SGCs. We found enriched expression of the tissue inhibitor metalloproteinase 3 (TIMP3) and other metalloproteinases in SGCs. Small interfering RNA and neutralizing antibody experiments revealed that TIMP3 modulates somatosensory stimuli. TIMP3 expression decreased after paclitaxel treatment, and its rescue by delivery of a recombinant TIMP3 protein reversed and prevented paclitaxel-induced pain. We also established that paclitaxel directly impacts metalloproteinase signaling in cultured SGCs, which may be used to identify potential new treatments for pain. Therefore, our results reveal a metalloproteinase signaling pathway in SGCs for proper processing of somatosensory stimuli and potential discovery of novel pain treatments.
科研通智能强力驱动
Strongly Powered by AbleSci AI