氧化还原
化学
黄素组
酶
脱氢酶
伤口愈合
电子传输链
电子转移
抗氧化剂
生物物理学
生物化学
光化学
有机化学
生物
免疫学
作者
Min Hu,Xia Wang,Yujing Tang,Xingyue He,Hongdou Shen,Hui Pan,Yinghui Shang,Dongbei Wu,Sheng‐Cai Zheng,Qigang Wang
出处
期刊:Nano Today
[Elsevier]
日期:2023-10-17
卷期号:53: 102028-102028
被引量:3
标识
DOI:10.1016/j.nantod.2023.102028
摘要
Redox homeostasis catalyzed by series oxidoreductases is important to maintain normal physiological functions, including mutually contradictory reactive oxygen species generation or reduction by separated enzyme regulation during cellular metabolism. The unidirectional enzyme regulation of redox homeostasis by biocatalytic reaction is an emerging catalytic medicine approach to disease biomimetic treatments. Bioinspired by photosynthesis, an efficient photo-enzyme-coupled hydrogel platform (Gel) with a spatio-temporally controlled hydrogel network was constructed through photoenzymatic-initiated radical polymerization in the absence of the corresponding enzyme substrate. Then, the coupling mechanism of light-induced electron transfer-enzyme activity center allosteric was explored through free radical analysis and theoretical calculations. The photo-switchable redox performance has been demonstrated based on the special anaerobic dehydrogenases, the flavin adenine dinucleotide (FAD)-centered dihydrolipoamide dehydrogenase (DLD) platform. At last, both in vitro and in vivo biological effects have been verified to evaluate the bidirectional oxidation/antioxidant modulation for the complicated wound healing in diabetic mice and wound-infected animals. The photoenzymatic coupling dehydrogenase-laden hydrogel platform proposed in this work provides ideas for the engineering design of enzymes, and the physical-biochemical regulatory mechanisms also offers a theoretical basis for controllable activation of enzyme activity, allowing for potential biomedical applications in metabolic processes.
科研通智能强力驱动
Strongly Powered by AbleSci AI