免疫分析
放射免疫分析
化学发光免疫分析
色谱法
化学发光
化学
抗体
生物
生物化学
免疫学
作者
Nobuyuki Amino,Yoh Hidaka
出处
期刊:PubMed
日期:1995-09-01
卷期号:53 (9): 2107-11
被引量:1
摘要
Five types of immunoassay, enzyme immunoassay (EIA), radioimmunoassay (RIA), fluoroimmunoassay (FIA), chemiluminescent immunoassay (CLIA) and counting immunoassay (CIA), are generally used. Radioimmunoassay was first developed but it needs specific facilities and the half life of radioisotope is not long. Enzyme immunoassay is at present most popular in Japan. The goal of development of immunoassay is improvement of sensitivity and automation. In order to improve sensitivity of an assay, immunoenzymometric assay (IEMA), which is a non-competitive method, was developed and fluorescent materials and chemiluminescent materials are used for the detection of enzyme activity. Automation of immunoenzymometric assay could be done with solid phase antibody method.
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