TISSUE CULTURE FROM VARIOUS EXPLANTS OF CHINESE CHESTNUT CULTIVARS

Eleven Chinese chestnut cultivars were selected for callus induction by tissue culture. The results showed that the better media for callus induction were WPM and MS media. 'Tanqiao', 'Xintian', 'Shao Shiba', 'Huaguan', and 'Hongli' were induced callus successfully. The induction rate, 75%, from embryo as the explant was higher than that of other tissues. The inducement rate from young stems was 65%. Both petioles and leaves as explants produced less callus. The better hormone combinations for some Chinese chestnut cultivars were 0.5 mg/L of 6-BA and 0.2 mg/L of NAA. Adventitious buds were induced from callus of 'Tangqiao' and roots could be produced from callus of 'Huaguan'.