泛素连接酶
泛素
隐色素
拟南芥
泛素蛋白连接酶类
生物
蛋白质水解
细胞生物学
蓝光
遗传学
生物化学
生物钟
基因
突变体
酶
物理
光学
作者
Yadi Chen,Xiaohua Hu,Siyuan Liu,Tianning Su,Hsiaochi Huang,Huibo Ren,Zeliang Gao,Xu Wang,Deshu Lin,James A. Wohlschlegel,Qin Wang,Chentao Lin
标识
DOI:10.1038/s41467-021-22410-x
摘要
Cryptochromes (CRYs) are photoreceptors or components of the molecular clock in various evolutionary lineages, and they are commonly regulated by polyubiquitination and proteolysis. Multiple E3 ubiquitin ligases regulate CRYs in animal models, and previous genetics study also suggest existence of multiple E3 ubiquitin ligases for plant CRYs. However, only one E3 ligase, Cul4COP1/SPAs, has been reported for plant CRYs so far. Here we show that Cul3LRBs is the second E3 ligase of CRY2 in Arabidopsis. We demonstrate the blue light-specific and CRY-dependent activity of LRBs (Light-Response Bric-a-Brack/Tramtrack/Broad 1, 2 & 3) in blue-light regulation of hypocotyl elongation. LRBs physically interact with photoexcited and phosphorylated CRY2, at the CCE domain of CRY2, to facilitate polyubiquitination and degradation of CRY2 in response to blue light. We propose that Cul4COP1/SPAs and Cul3LRBs E3 ligases interact with CRY2 via different structure elements to regulate the abundance of CRY2 photoreceptor under different light conditions, facilitating optimal photoresponses of plants grown in nature.
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