饱和突变
蛋白质工程
突变
计算生物学
蛋白质组
饱和(图论)
生物
计算机科学
遗传学
生物化学
突变体
基因
酶
数学
组合数学
作者
Marta M. Ferreira Amaral,Laura Frigotto,Anna V. Hine
出处
期刊:Methods in Enzymology
日期:2017-01-01
卷期号:: 111-133
被引量:2
标识
DOI:10.1016/bs.mie.2016.10.005
摘要
Beyond the natural proteome, high-throughput mutagenesis offers the protein engineer an opportunity to "tweak" the wild-type activity of a protein to create a recombinant protein with required attributes. Of the various approaches available, saturation mutagenesis is one of the core techniques employed by protein engineers, and in recent times, nondegenerate saturation mutagenesis is emerging as the approach of choice. This review compares the current methodologies available for conducting nondegenerate saturation mutagenesis with traditional, degenerate saturation and briefly outlines the options available for screening the resulting libraries, to discover a novel protein with the required activity and/or specificity.
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