[Detection Value of EB Virus DNA, IL-2, and IL-6 Level in Peripheral Blood of Children with Infectious Mononucleosis].

To investigative the detection value of EB virus DNA (EBV-DNA), interleukin-2 (IL-2), and interleukin-6 (IL-6) level in peripheral blood of children with infectious mononucleosis (IM).A total of 59 children clinically confirmed with IM in Anhui Provincial Children's Hospital from January 2018 to September 2020 were enrolled as IM group, while other 30 healthy children undergoing physical examination during the same period were enrolled as healthy group. The level of EBV-DNA load, IL-2, and IL-6 were compared between the two groups, and their diagnostic values for IM children were explored. According to the median level of EBV-DNA load, positive children were divided into high viral load group and low viral load group. The hepatomegaly and splenomegaly, and levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), IL-2, and IL-6 were compared between the two groups. The relationship between EBV-DNA load and IL-2, IL-6 levels were explored.The positive rate of EBV-DNA was 67.80% in IM group, which was significantly higher than 10.00% in healthy group (P<0.001), and the levels of serum IL-2 and IL-6 were also significantly higher than healthy group (P<0.001). The results of ROC curve analysis showed that AUC of IL-2 combined with IL-6 and EBV-DNA load was 0.948, which was significantly greater than that of IL-2, IL-6, and EBV-DNA load alone (0.847, 0.728, 0.789) (P<0.001). The cut-off value of IL-2 and IL-6 was 15.545 pg/ml and 56.560 pg/ml, respectively. Both the proportions of cases with moderate to severe hepatomegaly and splenomegaly in high viral load group were significantly higher than those in low viral load group (P<0.01, P<0.05). The levels of ALT, AST, and IL-2 in high viral load group were significantly higher than those in low viral load group (P<0.001), as well as IL-6 (P<0.01). In high and low viral load groups, EBV-DNA load was positively correlated with IL-2 and IL-6 (in high viral load group, rIL-2=0.598, rIL-6=0.416; in low viral load group, rIL-2=0.621, rIL-6=0.527, P<0.001).The detection of EBV-DNA load combined with IL-2 and IL-6 can improve the diagnostic accuracy of IM, and EBV-DNA load, IL-2 and IL-6 levels are related to the disease progression.传染性单核细胞增多症儿童外周血EB病毒DNA、IL-2 及IL-6水平的检测价值分析.探讨传染性单核细胞增多症（IM）儿童外周血EB病毒DNA（EBV-DNA）、白细胞介素-2（IL-2）及白细胞介素-6（IL-6）水平的检测价值.选择2018年1月至2020年9月在安徽省儿童医院确诊为IM的患儿59例作为IM组，选择同期来院体检的健康儿童30例作为健康组，比较两组EBV-DNA载量、IL-2及IL-6水平，探讨其对IM患儿的诊断价值，同时根据阳性患儿的EBV-DNA载量的中位水平，将其分为高病毒量组及低病毒量组，比较两组患者出现肝脏、脾脏肿大的情况和谷丙转氨酶（ALT）、谷草转氨酶（AST）及IL-2、IL-6水平，探究EBV-DNA载量与IL-2及IL-6水平的关系.IM组的EBV-DNA阳性率为67.80%，明显高于健康组的10.00%（P<0.001）；IM组的血清IL-2、IL-6水平亦明显高于健康组（P<0.001）。ROC曲线分析结果显示，3项指标联合的AUC为0.948，明显高于单一的IL-2、IL-6、EBV-DNA载量的AUC 0.847、0.728、0.789（P<0.001），同时IL-2、IL-6的cut-off值分别为15.545 pg/ml、56.560 pg/ml；高病毒量组患者的中重度肝、脾脏肿大人数明显高于低病毒量组（P<0.01，P<0.05）；高病毒量组的ALT、AST、IL-2水平均明显高于低病毒量组（P<0.001），且IL-6水平也高于低病毒量组（P<0.01）；在高、低病毒量组中，EBV-DNA载量与IL-2、IL-6均呈正相关（高病毒量组：rIL-2=0.598，rIL-6=0.416；低病毒量组：rIL-2=0.621， rIL-6=0.527，均P<0.001）.EBV-DNA载量联合IL-2、IL-6检测能够提高IM诊断的准确性，且EBV-DNA载量及IL-2、IL-6水平与患儿疾病进展相关.