小虾
环介导等温扩增
副溶血性弧菌
胶体金
病菌
肉眼
微生物学
检出限
生物
弧菌
食品科学
化学
DNA
材料科学
渔业
纳米技术
纳米颗粒
色谱法
细菌
生物化学
遗传学
作者
Yuling Sun,Chug‐Lun Lu,Chia‐Hung Lee,En‐I Tu,Chih‐Sheng Lin
摘要
Abstract Shrimp is one of the most important aquatic products in the world. Early detection, prevention, and treatment of shrimp pathogens is important. One major shrimp pathogen, Vibrio parahaemolyticus causes acute hepatopancreatic necrosis disease ( VP AHPND ) has been studied, but research into pathogen detection needs to be strengthened. Therefore, we were targeting to VP AHPND and using emerging molecular biotechnologies to develop a method for pathogens detection for the shrimp industry. Gold nanoparticles (AuNPs) have an unique color change property. Free AuNPs in solution are the color of claret‐red, while the aggregated AuNPs appear slight purple. Based on the characteristics of visible color difference of AuNPs, we worked on establishing a simple, fast, and specific loop‐mediated isothermal amplification (LAMP) detection method for VP AHPND diagnosis using AuNPs. This method, amplified the target DNA of VP AHPND by LAMP and the DNA were directly added onto AuNPs solution for detection. The test results show a color change, so that the results can be visually inspected by naked eye. Using the methods, shrimp VP AHPND can be early detected and treated, boost the economic benefits, and efficiency of shrimp farming.
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