Nickel oxide electroplating and electrode surface modification for electrochemical detection of glutamate

The electrochemical behavior and catalytic properties of nickel oxide (NiOx) are enhanced through cathodic electroplating and subsequent electrochemical activation in an alkaline aqueous electrolyte. The electrochemical detection of ʟ-glutamate becomes feasible by introducing an amine group through immobilizing APTES ((3-aminopropyl)triethoxysilane) on the NiOx surface, resulting in a positive charge. The synergistic effect of electrochemically activated electroplated NiOx and the APTES-modified surface facilitates the effective electrochemical detection of trace ʟ-glutamate. The APTES-activated NiOx electrode exhibits a linear detection range of 0.2 nM to 2 mM for glutamate. This relatively wide concentration range is promising for the analysis of human biological fluids.