Evaluation of Sensitive Urine DNA-Based PENK Methylation Test for Detecting Bladder Cancer in Patients with Hematuria

膀胱镜检查 尿 膀胱癌 金标准(测试) 医学 曲线下面积 甲基化 内科学 癌症 DNA甲基化 泌尿科 接收机工作特性 预测值 试验预测值 肿瘤科 泌尿系统 DNA 生物 基因 基因表达 遗传学
作者
Tae Jeong Oh,Ji‐Yong Lee,Yangyei Seo,Min A Woo,Jae Sung Lim,Yong Gil Na,Ki Hak Song,Bo‐Ram Bang,Justin Junguek Lee,Ju Hyun Shin,Sungwhan An
出处
期刊:The Journal of Molecular Diagnostics [Elsevier BV]
卷期号:25 (9): 646-654 被引量:6
标识
DOI:10.1016/j.jmoldx.2023.05.003
摘要

Hematuria is a prevalent symptom associated with bladder cancer (BC). However, the invasiveness and cost of cystoscopy, the current gold standard for BC diagnosis in patients with hematuria, necessitate the development of a sensitive and accurate noninvasive test. This study introduces and validates a highly sensitive urine-based DNA methylation test. The test improves sensitivity in detecting PENK methylation in urine DNA using linear target enrichment followed by quantitative methylation-specific PCR. In a case-control study comprising 175 patients with BC and 143 patients without BC with hematuria, the test's optimal cutoff value was determined by distinguishing between two groups, achieved an overall sensitivity of 86.9% and a specificity of 91.6%, with an area under the curve of 0.892. A prospective validation clinical study involving 366 patients with hematuria scheduled for cystoscopy assessed the test's performance. The test demonstrated an overall sensitivity of 84.2% in detecting 38 cases of BC, a specificity of 95.7%, and an area under the curve of 0.900. Notably, the sensitivity for detecting Ta high grade and higher stages of BC reached 92.3%. The test's negative predictive value was 98.2%, and the positive predictive value was 68.7%. These findings highlight the potential of the PENK methylation in urine DNA using linear target enrichment followed by quantitative methylation-specific PCR test in urine as a promising molecular diagnostic tool for detecting primary BC in patients with hematuria, which may reduce the need for cystoscopy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
汉堡包应助果粒多采纳,获得10
1秒前
5秒前
华仔发布了新的文献求助20
5秒前
5秒前
科研通AI2S应助杜杜采纳,获得10
7秒前
量子星尘发布了新的文献求助10
8秒前
JK发布了新的文献求助10
8秒前
打打应助顺利一德采纳,获得10
9秒前
法外狂徒完成签到,获得积分10
10秒前
Orange应助十九岁的时差采纳,获得10
10秒前
科研通AI2S应助steam采纳,获得10
12秒前
潇湘雪月发布了新的文献求助10
12秒前
13秒前
青青子衿完成签到,获得积分10
13秒前
13秒前
13秒前
15秒前
crazy发布了新的文献求助10
18秒前
杜杜发布了新的文献求助10
19秒前
嗯嗯发布了新的文献求助10
20秒前
老大蒂亚戈完成签到,获得积分10
22秒前
宝安完成签到,获得积分10
26秒前
JamesPei应助动听的老鼠采纳,获得10
26秒前
26秒前
杨可言完成签到,获得积分10
26秒前
27秒前
27秒前
28秒前
Hello应助子非鱼采纳,获得10
29秒前
30秒前
32秒前
mzhmhy发布了新的文献求助10
34秒前
李健的粉丝团团长应助ASA采纳,获得30
35秒前
Choi完成签到,获得积分0
35秒前
无辜如容发布了新的文献求助10
35秒前
123完成签到,获得积分10
36秒前
37秒前
单耳兔完成签到 ,获得积分10
37秒前
潇湘雪月发布了新的文献求助10
37秒前
故意的靳完成签到,获得积分10
39秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989263
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253814
捐赠科研通 3270066
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136