恒化器
大肠杆菌
灭菌(经济)
生物技术
发酵
重组DNA
生化工程
连续生产
食品科学
包涵体
生物
工艺工程
制浆造纸工业
生物化学
工程类
细菌
化学工程
业务
遗传学
基因
外汇市场
汇率
财务
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 87-102
标识
DOI:10.1007/978-1-0716-2930-7_6
摘要
Various fermentation strategies in industrial biotechnology are applied to produce recombinant target proteins using Escherichia coli. These proteins are often expressed as inclusion bodies (IBs), resulting in a high purity, high stability, and high product titers. In state-of-the-art fed-batch processes, product formation takes place in a short period of time. Sterilization, cleaning, and biomass growth are time consuming steps and reduce the space-time yield. Thus, the interest in establishing continuous cultivations, facilitating higher space-time yields, has been increased in recent years. In this protocol, we provide information and a guide to set-up the production of recombinant proteins in fed-batch, as well as in chemostat continuous cultivations using E. coli.
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