利奈唑啉
屎肠球菌
肠球菌
微生物学
生物膜
抗生素
联合疗法
化学
药理学
医学
生物
细菌
金黄色葡萄球菌
万古霉素
遗传学
作者
Qingru Chang,Huinan Chen,Yifan Li,Hai Lü,Zifeng Yang,Junfeng Zeng,Ping Zhang,Junwei Ge,Mingchun Gao
出处
期刊:Molecules
[MDPI AG]
日期:2023-11-16
卷期号:28 (22): 7630-7630
标识
DOI:10.3390/molecules28227630
摘要
Enterococci resistance is increasing sharply, which poses a serious threat to public health. Rhamnolipids are a kind of amphiphilic compound used for its bioactivities, while the combination of nontraditional drugs to restore linezolid activity is an attractive strategy to treat infections caused by these pathogens. This study aimed to investigate the activity of linezolid in combination with the rhamnolipids against Enterococcus faecium. Here, we determined that the rhamnolipids could enhance the efficacy of linezolid against enterococci infections by a checkerboard MIC assay, a time-kill assay, a combined disk test, an anti-biofilm assay, molecular simulation dynamics, and mouse infection models. We identified that the combination of rhamnolipids and linezolid restored the linezolid sensitivity. Anti-biofilm experiments show that our new scheme can effectively inhibit biofilm generation. The mouse infection model demonstrated that the combination therapy significantly reduced the bacterial load in the feces, colons, and kidneys following subcutaneous administration. This study showed that rhamnolipids could play a synergistic role with linezolid against Enterococcus. Our combined agents could be appealing candidates for developing new combinatorial agents to restore antibiotic efficacy in the treatment of linezolid-resistant Enterococcus infections.
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