Tools and techniques for the discovery of therapeutic aptamers: recent advances

适体 指数富集配体系统进化 范围(计算机科学) 计算机科学 鉴定(生物学) 风险分析(工程) 计算生物学 生物 医学 遗传学 生物化学 植物 核糖核酸 基因 程序设计语言
作者
Lili Ai,Xinyi Jiang,Kejing Zhang,Cheng Cui,Бо Лю,Weihong Tan
出处
期刊:Expert Opinion on Drug Discovery [Taylor & Francis]
卷期号:18 (12): 1393-1411 被引量:1
标识
DOI:10.1080/17460441.2023.2264187
摘要

ABSTRACTIntroduction The pursuit of novel therapeutic agents for serious diseases such as cancer has been a global endeavor. Aptamers characteristic of high affinity, programmability, low immunogenicity, and rapid permeability hold great promise for the treatment of diseases. Yet obtaining the approval for therapeutic aptamers remains challenging. Consequently, researchers are increasingly devoted to exploring innovative strategies and technologies to advance the development of these therapeutic aptamers.Areas covered The authors provide a comprehensive summary of the recent progress of the SELEX (Systematic Evolution of Ligands by EXponential enrichment) technique, and how the integration of modern tools has facilitated the identification of therapeutic aptamers. Additionally, the engineering of aptamers to enhance their functional attributes, such as inhibiting and targeting, is discussed, demonstrating the potential to broaden their scope of utility.Expert opinion The grand potential of aptamers and the insufficient development of relevant drugs have spurred countless efforts for stimulating their discovery and application in the therapeutic field. While SELEX techniques have undergone significant developments with the aid of advanced analysis instruments and ingeniously updated aptameric engineering strategies, several challenges still impede their clinical translation. A key challenge lies in the insufficient understanding of binding conformation and susceptibility to degradation under physiological conditions. Despite the hurdles, our opinion is optimistic. With continued progress in overcoming these obstacles, the widespread utilization of aptamers for clinical therapy is envisioned to become a reality soon.KEYWORDS: Therapeutic aptamersSELEX techniqueaptamer engineeringprotein degradationreceptor protein blockadetargeted therapy Article highlights The application of aptamers for therapeutic intentions reserves vast potential yet faces the lack of druggable candidates.The optimization of SELEX (Systematic Evolution of Ligands by EXponential enrichment) technique involved varying selection conditions and improving analysis methods, consequently, the promoted efficiency and applicability of SELEX boosted the identification of therapeutic aptamers.The therapeutic potency of aptamers is to some extent impeded by their relatively simple functions and limited working conditions. By leveraging facile engineering methods, aptamers could be endowed with versatile capabilities, such as acting as agonists or antagonists.The utilization of aptamers as targeting segment in therapeutic agents has exhibited promising effects, which facilitates the targeted delivery of chemotherapeutic drugs, nanostructures and immune cells to specific sites within the body.Tools and techniques for understanding the structure–activity relationships (SARs) of aptamers are still in their infancy. Dedicated researchers have the opportunity to drive forward the creation of relevant prediction and analysis approaches, which will undoubtedly aid in unlocking the full potential of aptamers for therapeutic applications.Declaration of interestThe authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.Reviewer disclosuresPeer reviewers on this manuscript have no relevant financial or other relationships to disclose.Additional informationFundingThis article was supported by the National Key Research and Development Program of China [2021YFA0910000].
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
9秒前
老张完成签到,获得积分10
15秒前
17秒前
zhugao完成签到,获得积分10
19秒前
22秒前
南风知我意完成签到,获得积分10
25秒前
朴实寻琴完成签到 ,获得积分10
25秒前
可可可爱完成签到 ,获得积分10
28秒前
lsy完成签到,获得积分10
32秒前
量子星尘发布了新的文献求助10
35秒前
36秒前
36秒前
hwen1998完成签到 ,获得积分10
39秒前
40秒前
41秒前
wwb发布了新的文献求助10
44秒前
45秒前
47秒前
LHT完成签到,获得积分10
48秒前
落寞凌波发布了新的文献求助10
52秒前
桐桐应助幸福的杨小夕采纳,获得10
57秒前
韩麒嘉完成签到 ,获得积分10
59秒前
聪慧的凝海完成签到 ,获得积分0
1分钟前
1分钟前
wwb发布了新的文献求助10
1分钟前
phil完成签到 ,获得积分10
1分钟前
1分钟前
高高菠萝完成签到 ,获得积分10
1分钟前
滴滴滴完成签到 ,获得积分10
1分钟前
yangsi完成签到 ,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
酷炫葵阴发布了新的文献求助10
1分钟前
ORANGE完成签到,获得积分10
1分钟前
思源应助松松采纳,获得20
1分钟前
共享精神应助酷炫葵阴采纳,获得10
1分钟前
丝丢皮得完成签到 ,获得积分10
1分钟前
1分钟前
xfy完成签到,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038029
求助须知:如何正确求助?哪些是违规求助? 3575740
关于积分的说明 11373751
捐赠科研通 3305559
什么是DOI,文献DOI怎么找? 1819224
邀请新用户注册赠送积分活动 892652
科研通“疑难数据库(出版商)”最低求助积分说明 815022