Screening for microplastics in drinking water and its toxicity profiling in zebrafish

Microplastics (MPs) have emerged as a major environmental problem in freshwater and marine environments. The effects of these polymers on aquatic life are well studied; however, there is limited knowledge of MP-associated health hazards in humans. We estimated the presence of MPs in different brands of bottled water available in India using the Nile red (NR) staining method. The FTIR examination revealed the presence of polystyrene (PS), polyethylene (PE), and polyamide (PA) in the bottled water samples with PE being the most prevalent one. Zebrafish embryos exposed to different concentrations of fluorescent-tagged polyethylene microplastics (PE-MPs) (10-150 μm) showed accumulation patterns at different time points in various organs. The exposure to PE MPs induced a concentration-dependent ROS activity. The expression of first-line antioxidative defense marker genes were significantly downregulated in embryos exposed to varying concentrations of PE-MPs, suggesting concentration and time-dependent effects on zebrafish. The results of this study suggest that the potential negative consequences on human health could be due to the oxidative stress and time-dependent toxicity of MPs.