小头畸形
病毒学
寨卡病毒
病毒
传染病(医学专业)
纳米技术
生物
疾病
医学
材料科学
病理
遗传学
作者
Yunxia Wu,Ronghua Yang,Qikang Wu,Mingxing Huang,Bowen Shu,Wenjie Wu,Baoqing Sun,Jinyu Xia,Xiaohong Chen,Yuhui Liao
出处
期刊:ACS omega
[American Chemical Society]
日期:2022-10-14
卷期号:7 (42): 37499-37508
被引量:1
标识
DOI:10.1021/acsomega.2c04280
摘要
Emerging infectious diseases have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) was globally spread and caused immeasurable damages. Thus, the monitoring of highly pathogenic viruses is important to prevent and control emerging infectious diseases. Herein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious diseases. A dendritic polymer probe was employed as the efficient signal emitter component that could generate an amplified ECL signal on the integrated chip, and the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy, the ZIKV in a complex system of blood, urine, and saliva was detected. The results indicated that a high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of the S and N protein gene sequence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples. Thus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical requirements for trace analysis of an emerging virus, and thus has the potential to serve as a paradigm for monitoring emerging infectious diseases.
科研通智能强力驱动
Strongly Powered by AbleSci AI