Occurrence and toxicity mechanisms of perfluorobutanoic acid (PFBA) and perfluorobutane sulfonic acid (PFBS) in fish

Perfluorobutanoic acid (PFBA) and perfluorobutane sulfonic acid (PFBS) are short-chain perfluoroalkyl substances (PFAS) ubiquitous in the environment. Here we review data on the presence and toxicity mechanisms of PFBA and PFBS in fish. We aimed to (1) synthesize data on physiological systems perturbed by PFBA or PFBS; (2) determine whether toxicity studies use concentrations reported in aquatic ecosystems and fish tissues; (3) conduct a computational toxicity assessment to elucidate putative mechanisms of PFBA and PFBS-induced toxicity. PFBA and PFBS are reported in the low ng/L in aquatic systems, and both substances are present in tissues of several fish including carp, bass, tilapia, and drum species. Evidence supports toxicity effects on several organ systems, including the cardiac, immune, hepatic, and reproductive system. Multigenerational effects in fish have also been documented for these smaller chain PFAS. To further elucidate mechanisms of reproductive impairment, we conducted in silico molecular docking to evaluate chemical interactions with several fish estrogen receptors, specifically zebrafish, fathead minnow, and Atlantic salmon. PFBS showed higher binding affinity for fish estrogen receptors relative to PFBA. Computational analysis also pointed to effects on lipids "Adipocyte Hypertrophy and Hyperplasia", "Lipogenesis Regulation in Adipocyte", and estrogen-related processes. Based on our review, most data for PFBA and PFBS are gathered for concentrations outside environmental relevance, limiting our understanding of their environment impacts. At the time of this review, there is relatively more toxicity data available for PFBS relative to PFBA in fish. This review synthesizes data on environmental levels and toxicology endpoints for PFBA and PFBS in fish to guide future investigations and endpoint assessments.